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KMID : 1101320050370010047
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Korean Journal of Clinical Laboratory Science
2005 Volume.37 No. 1 p.47 ~ p.55
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Comparative Research of Decalcification Methods for Quick Diagnosis on Bone Tissue
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Kim Sung-Chul
Back Oun-Chul
Kim Tai-Jeon
Bae Hyung-Joon
Kang Hee-Gyoo
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Abstract
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These studies were done to know decalcification methods to reduce the time of decalcification for quick bone tissue diagnosis. When bone tissue was decalcified with 10 % formic acid at room temperature, decalcification and hematoxylin & eosin (H&E) stains were complete and satisfactory after 12 hours, but some of the tissue sections fell off during staining. In this way, decalcification, H&E stains were complete and satisfactory after 24 hours, 36 hours and 48 hours, tissue sections didn't fall off during staining. When bone tissue was decalcified with 10 % formic acid in a 60 ℃ paraffin oven, decalcification and H&E stains were complete and satisfactory after 6 hours, but some tissue sections fell off during staining. In this way, decalcification and tissue sections were complete, with no falling off during staining after 8 hours, 10 hours, 12 hours, 14 hours, 24 hours, or H&E stains were satisfactory from 8 hours to 12 hours, but H&E stains appeared to reddish nucleus after 14 hours and 24 hours. Bone tissue was decalcified with 10 % formic acid for 6 hours, 12 hours and 24 hours at DECAL machine frequencies of 15 Hz and 45 Hz, and for 6 hours, 12 hours and 24 hours at a DECAL machine frequency of 90 Hz. Decalcification and H&E stains were complete and satisfactory after 6 hours at the 15 Hz and 45 Hz DECAL settings. Some of the tissue sections fell off during staining at the 15 Hz DECAL machine setting. At the 90 Hz setting, decalcification, H&E stains, and tissue sections were complete and satisfactory with no falling off during staining after 4 hours. In this way, decalcification, H&E stains, and tissue section were complete and satisfactory with no falling off during staining after 12 hours, 24 hours at all machine settings. Bone tissue was decalcified with 10 % formic acid for 6 hours, 12 hours and 24 hours at 37 ℃ 3 hours, 6 hours and 12 hours at 45 ℃ and 1 hours, 5 hours and 10 hours at 60 ℃ with the RHS-1 machine setting at 60Hz. At the temperatures of 37 ℃, 45 ℃, and 60 ℃ decalcification, H&E stains, and tissue sections were complete and satisfactory, with no falling off during staining except for after 6 hours at 37 ℃. 3 hours, 1 hours, or decalcification, H&E stains, and tissue sections were complete and satisfactory with no falling off during staining after 12 hours and 24 hours at 37 ℃, 6 hours and 12 hours at 45 ℃, and 5 hours at 60 ℃. But H&E stains appeared to reddish nucleus after 10 hours at 60 ℃. From the above reults, the authors were able to deduce that decalcification is accelerated by heat and frequency. We therefore think that it is necessary for machines which are similar to the RHS-1 machine to be maintained at the temperature evenly with agitation effect for quick decalcification.
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KEYWORD
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Decalcification, Formic acid, Decal machine, RHS-1 machine
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